A Research review by Sun et al. đ
đ
Mass spectrometry imaging (MSI) is an advanced analytical technique that combines mass spectrometry with spatial mapping, enabling the direct, label-free detection and visualization of molecular distributions within biological tissues. This review comprehensively outlines the fundamental principles, major technological platforms, and recent applications of MSI in plant science. We detail key ionization techniques â matrix-assisted laser desorption/ionization (MALDI), desorption electrospray ionization (DESI), and secondary ion mass spectrometry (SIMS) â focusing on their ionization mechanisms and instrumental characteristics.







