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Researchers develop method for deciphering positional rules in splicing

Posted in biological

A research team led by Prof. Xue Yuanchao from the Institute of Biophysics of the Chinese Academy of Sciences has developed a new method for global profiling of in-situ RNA–RNA contacts associated with a specific RNA-binding protein (RBP) and revealed positional mechanisms by which PTBP1-associated RNA loops regulate cassette exon splicing.

This study was published online in Molecular Cell on March 22.

In , the same pre-mRNA can produce multiple protein isoforms to execute similar or different biological functions through . Several longstanding models proposed that RBPs may regulate alternative splicing by modulating long-range RNA–RNA interactions (RRI). However, direct experimental evidence was lacking.