Lifeboat Foundation

Ineurals — advanced neuro-technologies for rapid learning and skill acquisition.

The 711th Human Performance Wing, under the U.S. Air Force Research Laboratory leads the development, integration, and delivery of Airman-centric research, education, and consultation enabling the U.S. Air Force to achieve responsive and effective global vigilance, global reach, and global power now and in the future. It’s comprised of the United States Air Force School of Aerospace Medicine and the Airman Systems Directorate, whose science and technology competencies include Training, Adaptive Warfighter Interfaces, Bioeffects, Bioengineering, and Aerospace and Operational Medicine.

Continue reading “INeuraLS — Advanced NeuroTech For Rapid Knowledge and Skill Acquisition — US AirForce Research Labs” »

Synthetic biology tools used to engineer T cells that work like living computers and recognize antigen combinations in solid tumors.

Although Drosophila is an insect whose genome has only about 14,000 genes, roughly half the human count, a remarkable number of these have very close counterparts in humans; some even occur in the same order in the fly’s DNA as in our own. This, plus the organism’s more than 100-year history in the lab, makes it one of the most important models for studying basic biology and disease.

To take full advantage of the opportunities offered by Drosophila, researchers need improved tools to manipulate the fly’s genes with precision, allowing them to introduce mutations to break genes, control their activity, label their protein products, or introduce other inherited genetic changes.

“We now have the genome sequences of lots of different animals — worms, flies, fish, mice, chimps, humans,” says Roger Hoskins of Berkeley Lab’s Life Sciences Division. “Now we want improved technologies for introducing precise changes into the genomes of lab animals; we want efficient genome engineering. Methods for doing this are very advanced in bacteria and yeast. Good methods for worms, flies, and mice have also been around for a long time, and improvements have come along fairly regularly. But with whole genome sequences in hand, the goals are becoming more ambitious.”

O,.o Circa 2019

CRISPR/Cas9 is now a household name associated with genetic engineering studies. Through cutting-edge research described in their paper published in Scientific Reports, a team of researchers from Tokyo University of Science, Meiji University, and Tokyo University of Agriculture and Technology, led by Dr Takayuki Arazoe and Prof Shigeru Kuwata, has recently established a series of novel strategies to increase the efficiency of targeted gene disruption and new gene “introduction” using the CRISPR/Cas9 system in the rice blast fungus Pyricularia (Magnaporthe) oryzae. These strategies include quicker (single-step) gene introduction, use of small homologous sequences, and bypassing of certain prerequisite host DNA “patterns” and host component modification.

The team led by Dr Arazoe and Prof Kuwata has devised simple and quick techniques for gene editing (target gene disruption, sequence substitution, and re-introduction of desired genes) using CRISPR/Cas9 in the rice blast fungus Pyricularia (Magnaporthe) oryzae, a type of filamentous fungus. Spurred on by encouraging results, the researchers surmise, “Plants and their pathogens are still coevolving in nature. Exploiting the mutation mechanisms of model pathogenic fungi as a genome editing technique might lead to the development of further novel techniques in genetic engineering.”

The working component of the CRISPR/Cas9 system binds to the target gene region (DNA) and causes a site-specific double-stranded break (DSB) in the DNA. Effective binding of this component requires a certain “motif” or “pattern” called the protospacer-adjacent motif (PAM), which follows downstream of the target gene region.

The honeybee (Apis mellifera) is an important insect pollinator of wild flowers and crops, playing critical roles in the global ecosystem. Additionally, the honeybee serves as an ideal social insect model. Therefore, functional studies on honeybee genes are of great interest. However, until now, effective gene manipulation methods have not been available in honeybees. Here, we reported an improved CRISPR/Cas9 gene-editing method by microinjecting sgRNA and Cas9 protein into the region of zygote formation within 2 hr after queen oviposition, which allows one-step generation of biallelic knockout mutants in honeybee with high efficiency. We first targeted the Mrjp1 gene. Two batches of honeybee embryos were collected and injected with Mrjp1 sgRNA and Cas9 protein at the ventral cephalic side and the dorsal posterior side of the embryos, respectively. The gene-editing rate at the ventral cephalic side was 93.3%, which was much higher than that (11.8%) of the dorsal-posterior-side injection. To validate the high efficiency of our honeybee gene-editing system, we targeted another gene, Pax6, and injected Pax6 sgRNA and Cas9 protein at the ventral cephalic side in the third batch. A 100% editing rate was obtained. Sanger sequencing of the TA clones showed that 73.3% (for Mrjp1) and 76.9% (for Pax6) of the edited current-generation embryos were biallelic knockout mutants. These results suggest that the CRISPR/Cas9 method we established permits one-step biallelic knockout of target genes in honeybee embryos, thereby demonstrating an efficient application to functional studies of honeybee genes. It also provides a useful reference to gene editing in other insects with elongated eggs.

Initially discovered in bacteria, CRISPR-based genome editing endonucleases have proven remarkably amenable for adaptation to insects. To date, these endonucleases have been utilized in a plethora of both model and non-model insects including diverse flies, bees, beetles, butterflies, moths, and grasshoppers, to name a few, thereby revolutionizing functional genomics of insects. In addition to basic genome editing, they have also been invaluable for advanced genome engineering and synthetic biology applications. Here we explore the recent genome editing advancements in insects for generating site-specific genomic mutations, insertions, deletions, as well as more advanced applications such as Homology Assisted Genome Knock-in (HACK), potential to utilize DNA base editing, generating predictable reciprocal chromosomal translocations, and development gene drives to control the fate of wild populations.

Sneezes from people who have congested noses and a full set of teeth travel about 60% farther than from people who don’t, according to a new study.

New research from the University of Central Florida has identified physiological features that could make people super-spreaders of viruses such as COVID-19.

In a study appearing this month in the journal Physics of Fluids, researchers in UCF’s Department of Mechanical and Aerospace Engineering used computer-generated models to numerically simulate sneezes in different types of people and determine associations between people’s physiological features and how far their sneeze droplets travel and linger in the air.

In a new realm of materials, PhD student Thanh Nguyen uses neutrons to hunt for exotic properties that could power real-world applications.

Thanh Nguyen is in the habit of breaking down barriers. Take languages, for instance: Nguyen, a third-year doctoral candidate in nuclear science and engineering (NSE), wanted “to connect with other people and cultures” for his work and social life, he says, so he learned Vietnamese, French, German, and Russian, and is now taking an MIT course in Mandarin. But this drive to push past obstacles really comes to the fore in his research, where Nguyen is trying to crack the secrets of a new and burgeoning branch of physics.

“My dissertation focuses on neutron scattering on topological semimetals, which were only experimentally discovered in 2015,” he says. “They have very special properties, but because they are so novel, there’s a lot that’s unknown, and neutrons offer a unique perspective to probe their properties at a new level of clarity.”

A landmark study shows this age-old tech is the key.

The cure for aging has long been the Holy Grail of medicine. Emerging technologies, like the gene editing tool CRISPR, have opened the floodgates to what may be possible for the future of medical science. The key to slowing down aging, however, may lie in a simple and age-old technique.

Dive deeper. ➡ Read best-in-class health, tech, and science features, and get unlimited access to Pop Mech.

Continue reading “It Sure Looks Like Humans Have Found a Way to Reverse Aging” »