Circa 2008
From endpoint fluorescence to melting curve analysis.
Today’s research on somatic, genetic and epigenetic variation in eukaryotic cells requires fast, accurate and cost-effective methods for screening large numbers of samples or loci in parallel. Variations identified by genomic sequencing or array studies need to be subsequently confirmed and validated.
Real-time PCR has become a well established technology for this purpose. The plate-based LightCycler 480 system offers a broad selection of methods and applications (Fig. 1).